Anti-bacterial activity of coumarin enriched extract from Cyperus rotundus rhizomes against Urinary Tract Infection

Abstract

Rhizomes of Cyperus rotundus have been traditionally used for several ailments, particularly in urinary tract infection and inflammation. This study aims to determine the antibacterial activity of coumarin enriched Cyperus rotundus extract in inhibiting the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus and Escherichia coli. The FT-IR spectra of root extracts revealed the presence of functional groups characteristic of alcohols, carboxylic acids, aromatic compounds, alkanes, alkenes, and amines. The GC-MS investigation led to the identification of diverse phytoconstituents. Further, various concentrations of C. rotundus extract at 500 µg/ml, 250 µg/ml, 100 µg/ml and 50 µg/ml was studied and compared with Gentamycin as standard. The results showed that the extract of C. rotundus could inhibit the growth of microorganisms with the high inhibition zone as indicated by a concentration of 500 µg/ml. Thus, the results of the present study will create a way for the invention of plant-based medicines for various microbial infections using C. rotundus which may lead to the development of novel drugs against drug-resistant microbial infections.