Evaluation of pharmacological activity of Artemisia pallensby Antibacterial and Antioxidant activity: An invitro study

Abstract

Plants are a rich source of therapeutic compounds that have tremendous applications in the pharmaceutical industry. Artemisia pallens is a plant that has long been utilized in traditional medicine as a treatment for certain diseases. This study aims to analyse the phytochemicals as plant secondary metabolites, evaluate the antioxidant and antibacterial activities of A. pallensespecially in the four different districts of Karnataka region.Phytochemical analysis was performed to investigate the plant’s secondary metabolites such as Saponins, Flavonoids, Cardiac Glycosides, Terpenoids, Alkaloids, Steroids and Carbohydrates. The methanol extracts of the plants were used to evaluate the in vitro antioxidant activity by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical and the antibacterial activity against gram positive and gram-negative bacteria. Leaf extract A. pallens was observed to have the highest antioxidant activity with IC50 value as compared to the standard Butylated Hydroxy Toluene (BHT). The leaf extracts of four different regions also showed good potential zone of inhibition against Escherichia coli and Pseudomonas syringae which are Gram negative in nature. Only two samples showed good zone of inhibition against Bacillus subtilis and Staphylococcus aureus which are Gram positive in nature. Methanol extract ofA. pallens showed the best 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity. Two samples showed that the leaf extracts have the proton donating ability and could serve as free radical inhibitors or scavenging, acting possibly as primary antioxidants pallens extracts contain phytochemicals which are capable of mitigating against chronic health conditions such as cancer.