Single-cell RNA Sequencing for Identifying Tumour Micro Environment Heterogeneity in Breast Cancer

Abstract

Breast cancer is a heterogeneous disease characterized by diverse molecular subtypes and varying responses to treatment. The tumor microenvironment (TME) plays a critical role in tumor progression, immune evasion, and therapy resistance. Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for elucidating the complexity of the TME by providing high-resolution insights into the gene expression profiles of individual cells. This paper reviews the application of scRNA-seq in breast cancer research, focusing on its ability to dissect TME heterogeneity, identify novel cell types and states, and uncover potential therapeutic targets.