An interactive effect of Diosmin and High-intensity interval training on gene expression and activity of antioxidant enzymes in patients with type 2 diabetes

Abstract

Background: There is strong evidence that the development of type 2 diabetes (T2DM) is significantly influenced by elevated oxidative stress. Despite the fact that earlier research has demonstrated the benefits of high-intensity interval training (HIIT) for T2DM, But no human study has yet looked into how Diosmin affects T2DM. Given that the redox status of patients with type-2 diabetes is significantly impacted by exercise and the ingestion of medicinal plants with antioxidant qualities, This study's objective was to determine how 12 weeks of high-intensity interval training (HIIT) and ingestion of Diosmin affected the gene expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in patients with type 2 diabetes.

Methods: Based on the inclusion criteria, 93 overweight (BMI > 25) T2DM patients were chosen to participate in this randomized, double-blind study. They were divided into four groups at random and placed in each for 12 weeks: Diosmin (DIS, N = 23), HIIT + Diosmin (Tr + DIS, N = 24), HIIT + placebo (Tr + PL, N = 23), and placebo (PL, N = 23). Before and after participation in all groups, blood samples were taken from every person to measure biochemical markers like lipid profile analysis, fasting blood glucose (FBG), insulin, glycated hemoglobin (HbA1c), and the homeostasis model of assessment-insulin resistance (HOMA-IR). Blood tests using real-time PCR are used to detect the expression of RCT elements.

Results: The interaction between HIIT and Diosmin ingestion significantly boosted CAT, SOD, and GPx gene expression and activity. Additionally, the effect of HIIT alone dramatically elevated SOD and CAT gene expression. However, Diosmin alone had no discernible effects on the expression of the GPx gene. All intervention groups experienced a substantial decrease in serum levels of glucose, HbA1c, HOMA-IR index, TC, and TG compared to the corresponding levels in the control group.

Conclusions: Based on the findings of this study, HIIT with a Diosmin supplement can enhance antioxidant and metabolic conditions. However, using a Diosmin supplement with HIIT at the same time can have beneficial synergistic benefits.