Optimising CRISPR-Cas9 for Targeted Cancer Therapy: Techniques and Challenges

Abstract

Globally, cancer is the second most common cause of mortality and continues to present significant social and economic challenges. Despite the fact that we are gaining a deeper understanding of the molecular causes of cancer, there is still a need for additional research to be conducted in order to create new therapeutic approaches and technologies that leverage these discoveries. The CRISPR-Cas9 genome editing technique has gained recognition in the field of cancer treatment due to its exceptional efficiency and accuracy. In numerous disciplines, including the investigation of chemical-genetic interactions and the identification of novel cancer treatment targets, CRISPR-Cas9 has demonstrated remarkable therapeutic potential. This technology offers critical new insights into the manner in which malignancies respond to medication treatment. Additionally, CRISPR-Cas9 can be employed in cancer immunotherapy to rapidly modify immune cells and oncolytic viruses. CRISPR-Cas9's most critical attribute is its precise gene editing capabilities, which extend beyond human tissue and cell culture models and can be employed for therapeutic research. This study examines critical factors that must be taken into account when using CRISPR/Cas9 in therapeutic applications, as well as significant obstacles that must be overcome prior to its clinical application in the treatment of a genetically influenced and intricate disease such as cancer.